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KMID : 0352720090330020143
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Journal of Ginseng Research
2009 Volume.33 No. 2 p.143 ~ p.148
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Optimization of Submerged Culture Conditions for the Growth Increase of Ginseng Adventitious Root Containing Germanium
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Chang Eun-Jung
Oh Hoon-Il
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Abstract
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This study was carried out to determine the optimal submerged culture conditions for the growth increase of ginseng adventitious roots containing germanium by means of a fractional factorial design with four factors and three levels, using the response surface methodology (RSM). The ginseng (Panax ginseng C.A. Meyer) adventitious roots were induced by plant growth regulators and cultured in a liquid SH medium. The effects of various GeO₂ and phosphoric acid (H₃PO₄) concentrations in the medium, GeO₂ addition time and the pH of the medium on the fresh weight of the ginseng adventitious roots were investigated. The optimum pH of the medium and the phosphoric acid concentration determined by the partial differentiation of the model equation were 4.7 and 6.0 mM, respectively. The predicted optimal GeO₂ concentration was 10 ppm and the GeO₂ addition time did not affect the growth of ginseng adventitious roots. Under these conditions, the growth of the ginseng adventitious root containing germanium was predicted to be 2.47 g.
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KEYWORD
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Ginseng, adventitious root, germanium, fresh weight, response surface methodology
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